CO 2 & O 2 control. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). * Note: Programming the washer with a final aspirate height of 4. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Endpoint, kinetic, spectrum. ~ Bio-Tek ELx808 Microplate Reader $2,795. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Corning. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. #C1500) Control standard endotoxin (ACC cat. 96-well-plate-scan-time-2-colors-1-site-well. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. 1% in white 96- and < 0. and glow luminescence modes in 96- to 384-well plates. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. UV absorbance detection. with Quartz ABS Plastic. Detection of KASP genotyping reactions. Your applications, your modes, your choice. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Return to top. The variations include adjustable. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. Including injection time, it takes approximately 68 secs. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Compare Tool. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. 3) At the bottom of the strip, click on "Show. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. ZERO BIAS - scores, article reviews, protocol conditions and moreThe VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Materials. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Published Dec 16, 2020 | Updated Dec 16,. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Unparalleled performance on a personalized platform. Z. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. minutes and begin reading microplates with seven mouse clicks. ©2022 Molecular Devices - All rights. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. 96-, 384-, and 1536-well microplates. White plates reflect the light, enabling even low levels of Luminescence to be detected. Cell viability and cytoxicity assays. Tristar 3 Multimode Microplate Reader. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 100 μL of working reagent was added to each well after the 10-minute preincubation step. A control well was set up to verify. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. PURPOSE. 3 OD at 200 nm. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. 313 reference gain check fail. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Molecular Devices SpectraMax Plus 384 Absorbance. (96- and 384-well with pipetting) 6-384 (96- and 384-well). SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. High-throughput, automated dynamic light scattering. 96-well plates - Filter plates with 0. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 0 . A standard 96 well plate was set up as described in Protocol Table 4. $3,207. Includes PC and software. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. 00. 312 gain calibration failed. SpectraMax 250 User. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Temperature variation ± 0. Features. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. $488. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Pick the best speed, sensitivity, and price for your needs. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. x. Infinite possibilities. M2. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Bay Gray powder coated metal back plate, column, and base. 5% at 1. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Two different agonists, acetylcholine and carbachol, were tested. Description. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. x. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. The device was able to achieve 3σ limits of detection of ca. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. Page 1 of 8 . S. Get Quote . Optimized for fluorescence microplate readers. Cayman Chemical. for molecular biology. Step 2: Launch SoftMax Pro Software. President of Molecular Devices. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. 340 PC384. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Absorbance Linearity: 2. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. p-Nitroaniline - Catalog No. 96- or 384-well microplate compatibility. Microplate Spectrophotometers. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. x. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. (one set per assay) to clear 96-well microplates. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. For biochemical assays, the washer removes unbound material and unreacted reagents. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Reads various kinds of 96-wells plates, and is equipped with shaking function. Optional 16-way manifold. 305 reference level saturation. Polypropylene deep well plates, round bottom. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. No accessories are needed and SoftMax® Pro Software. 3. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Published Dec 16, 2020 | Updated Dec 06, 2021. 315 can't find zero order. x. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. The VICTOR Nivo. $14,995. • Photometric range: 0. Our microplate. Can read 96 well plates (optional 384 well). 5in (46. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Compare this item. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. 2. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. . 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. Temperature control: 4°C above ambient to 45°C. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Or scan up to 384 wells at any time to determine the best assay conditions. 190. 6. 039. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. measurements in compatible Molecular Devices® microplate readers. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. x. M2. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. ₹ 1,80,000 Get Latest Price. $542. µl) was added to each well and incubated at 37°C for 1 h. Users can choose to process all plates within the magazine or select a specific number. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Wells to read. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. The system can perform readings. 100 fmol luciferase per well. Other technologies such as imaging. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. Service Contracts, Preventative Maintenance and. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Published Dec 16, 2020 | Updated Dec 16, 2020. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. Learn how you can utilize microplate readers to drive landmark discoveries. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Populate the following info under Description. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Multimode microplate reader equipped with latest detections like fluorescence,. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. These robust readers have been placed in labs from Antarctica to. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. Compare this item. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. com. SpectraDrop High-Throughput Screening (HTS) Kit. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Part of a plate can be selected so that only a selected area of the plate. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. x. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Plus 384. Software Validation. 1. The soil was amended with either 1. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. This page contains the. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. No. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). 306 plate air cal. as well as all other Molecular Devices' microplate readers. Component A (2 vials*)Figure 1. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Description. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. • 0. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. variety of. QuickSync. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. $3,450. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Features. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Intuitive graphical user interface makes each assay extremely easy to set. These microplates have a corner notch for use in standard plate readers. • Easy and gentle immobilization of biotin. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Plate chamber design of the SpectraMax category 1 microplate readers. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. d. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Other. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Dark blue indicates well will be read. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. 2% in. Time-resolved phosphorescence. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Uncontrolled Copy for Training and Reference Purposes Only . Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 96-, 384-, and 1536-well microplates. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. It also integrates with the powerful SoftMax Pro Software for. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. #E010) Pyroplate 96-well microplate (ACC cat. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Our. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. 9. The IC50s by cell rounding and LysoTracker. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. 0 . : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. 1 fmol luciferase per well. Published Dec 16, 2020 | Updated Dec 16, 2020. #CA961) Methods. Selects either 96-well or 384-well plate for a stand-alone read. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 96 well plate injection time with SpectraMax® L plate reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. FlexIII. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. Make sure toPlate reader instructions Computer. UV Plate 96 Well, Flat Bottom, Cs 50. SpectraMax i3x Multi-Mode Microplate Reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. D. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 2b,c. Multimodal plate reader: Yes: 25 mm: Yes—Max. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. style. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. Instrument settings can. When a. 300 µL of Detergent Compatible Bradford reagent was added to each well. External Dimensions without lid (mm). By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. Plate. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. U-bottom: 30-40 µl. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Uncontrolled Copy for Training and Reference Purposes Only . SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Reader Documentation Version No. We would like to show you a description here but the site won’t allow us. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. The CellTiter-Glo assay was used to measure ATP. Another consideration, says Amouretti, is plate format. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 9. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Step 3: Click “Settings” and a screen will appear (Figure 1). The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Create custom fluorescence assays without having to purchase expensive filter sets. 00. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 0200-6262. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 25% and 0. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. a white 96-well half-area microplate (Corning cat. Instrument settings can. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. 1. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. • High antibody-binding surface improves the overall performance in. Next-generation luminescence plate reader. C. Top or bottom detection is available with a simple click of a button. Inconsistent results: cannot determine whole well confluence – well after well. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below.